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KMID : 0350519920450030973
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Journal of Catholic Medical College
1992 Volume.45 No. 3 p.973 ~ p.987
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c-myc DNA Ampilfication and DNA Ploidy Analysis in Cervical Cancer of Uterus
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Abstract
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We conducted this experiment to find out the possibility that DNA patterns obtained by flow cytometry and the presence or absence of c-myc DNA amplification in patients with cervical cancer can be used as a biological prognostic factors.
We studied 31 cervical cancer patients with clinical stage I or II who visited the Clinic of Cervical Neoplasia at Department of Obstetrics and Gynecology, Catholic University Medical College from Jan. 1991 to Dec, for whom radical hysterectomy
were
performed.
We obtained following results from those patients by analyzing the results and factors such as patterns of c-myc DNA amplification, detection of Human papillomavirus (HPV) subtype, DNA analysis obtained form flow cytometry, clinical stage, age
histologic findings.
1. The incidence of c-myc DNA amplification was significantly higher in group with lymphovascular involvement than those group without lymphovascular involvement, but the amplification rate of c-myc DNA was not affected by age, clinical stage,
cell
type, histologic differentiation, tumor size, depth of invasion, and pelvic lymph node metastasis.
2. DNA index was significantly higher in non-keratinizing group than that of keratinizing group, but the mean S-phase rate was not significantly different between two groups. The value of DNA index was not significantly affected by other
pathologic
characteristics.
3. There was no significant difference in DNA index between the group with c-myc DNA amplification and the group without c-myc DNA amplification.
4. The incidence rate of aneuploid and diploid tumors was irrespective of prognostic factors classified by clinicopathologic characteristics.
5. The group which consisted of 6 cases(19.4%) with negative c-myc DNA amplification and diploid was significantly lower than other three groups which consisted of 25 cases(80.6%).
6. All 5 cases with detection of HPV subtype 16 showed c-myc DNA amplification patterns. From these results, we concluded that the pattern of c-myc DNA amplification and ploidy of cervical cancer may be promised as a prognostic index but further
follow
up study over a long period will be needed for clinical application.
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KEYWORD
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